Genome sequence of Ensifer sp. TW10; a Tephrosia wallichii (Biyani) microsymbiont native to the Indian Thar Desert. Issue 2 (September 2013)

• Record Type: Journal Article
• Authors: Tak, Nisha; Gehlot, Hukam; Kaushik, Muskan; Choudhary, Sunil; Tiwari, Ravi; Tian, Rui; Hill, Yvette; Bräu, Lambert; Goodwin, Lynne; Han, James; Liolios, Konstantinos; Huntemann, Marcel; Palaniappan, Krishna; Pati, Amrita; Mavromatis, Konstantinos; Ivanova, Natalia; Markowitz, Victor; Woyke, Tanja; Kyrpides, Nikos; Reeve, Wayne
• Is Part Of: Standards in genomic sciences. Volume 9:Issue 2(2013)
• Page Start: 304
• Page End: 314
• Publication Date: 2013-09
• Subjects: root-nodule bacteria -- nitrogen fixation -- rhizobia -- Alphaproteobacteria; Genomics -- Periodicals; Genomics -- Periodicals; Genomics; Periodicals; 572.8605
• DOI: 10.4056/sigs.4598281
• Languages: English
• ISSNs: 1944-3277

Abstract

Abstract Ensifer sp. TW10 is a novel N2 -fixing bacterium isolated from a root nodule of the perennial legumeTephrosia wallichii Graham (known locally as Biyani) found in the Great Indian (or Thar) desert, a large arid region in the northwestern part of the Indian subcontinent. Strain TW10 is a Gram-negative, rod shaped, aerobic, motile, non-spore forming, species of root nodule bacteria (RNB) that promiscuously nodulates legumes in Thar Desert alkaline soil. It is fast growing, acid-producing, and tolerates up to 2% NaCl and capable of growth at 40o C. In this report we describe for the first time the primary features of this Thar Desert soil saprophyte together with genome sequence information and annotation. The 6, 802, 256 bp genome has a GC content of 62% and is arranged into 57 scaffolds containing 6, 470 protein-coding genes, 73 RNA genes and a single rRNA operon. This genome is one of 100 RNB genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia forBacteria andArchaea -Root Nodule Bacteria (GEBA-RNB) project.