Genome sequence of Ensifer medicae strain WSM1115; an acid-tolerant Medicago-nodulating microsymbiont from Samothraki, Greece. Issue 3 (November 2014)

• Record Type: Journal Article
• Authors: Reeve, Wayne; Ballard, Ross; Howieson, John; Drew, Elizabeth; Tian, Rui; Bräu, Lambert; Munk, Christine; Davenport, Karen; Chain, Patrick; Goodwin, Lynne; Pagani, Ioanna; Huntemann, Marcel; Mavrommatis, Konstantinos; Pati, Amrita; Markowitz, Victor; Ivanova, Natalia; Woyke, Tanja; Kyrpides, Nikos
• Is Part Of: Standards in genomic sciences. Volume 9:Issue 3(2013)
• Page Start: 514
• Page End: 526
• Publication Date: 2014-11
• Subjects: root-nodule bacteria -- nitrogen fixation -- rhizobia -- Alphaproteobacteria; Genomics -- Periodicals; Genomics -- Periodicals; Genomics; Periodicals; 572.8605
• DOI: 10.4056/sigs.4938652
• Languages: English
• ISSNs: 1944-3277

Abstract

Abstract Ensifer medicae strain WSM1115 forms effective nitrogen fixing symbioses with a range of annualMedicago species and is used in commercial inoculants in Australia. WSM1115 is an aerobic, motile, Gram-negative, non-spore-forming rod. It was isolated from a nodule recovered from the root of burr medic (Medicago polymorpha ) collected on the Greek Island of Samothraki. WSM1115 has a broad host range for nodulation and N2 fixation capacity within the genusMedicago, although this does not extend to all medic species. WSM1115 is considered saprophytically competent in moderately acid soils (pH(CaCl2 ) 5.0), but it has failed to persist at field sites where soil salinity exceeded 10 ECe (dS/m). Here we describe the features ofE. medicae strain WSM1115, together with genome sequence information and its annotation. The 6, 861, 065 bp high-quality-draft genome is arranged into 7 scaffolds of 28 contigs, contains 6, 789 protein-coding genes and 83 RNA-only encoding genes, and is one of 100 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia forBacteria andArchaea -Root NoduleBacteria (GEBA-RNB) project.