High-quality permanent draft genome sequence of Ensifer medicae strain WSM244, a microsymbiont isolated from Medicago polymorpha growing in alkaline soil. Issue 1 (December 2015)

• Record Type: Journal Article
• Authors: Ardley, Julie; Tian, Rui; O'Hara, Graham; Seshadri, Rekha; Reddy, T.; Pati, Amrita; Woyke, Tanja; Markowitz, Victor; Ivanova, Natalia; Kyrpides, Nikos; Howieson, John; Reeve, Wayne
• Is Part Of: Standards in genomic sciences. Volume 10:Issue 1(2015)
• Page Start: 1
• Page End: 8
• Publication Date: 2015-12
• Subjects: Root-nodule bacteria -- Nitrogen fixation -- Symbiosis -- Alphaproteobacteria -- Ensifer -- GEBA-RNB; Genomics -- Periodicals; Genomics -- Periodicals; Genomics; Periodicals; 572.8605
• DOI: 10.1186/s40793-015-0119-5
• Languages: English
• ISSNs: 1944-3277

Abstract

Abstract Ensifer medicae WSM244 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont ofMedicago species. WSM244 was isolated in 1979 from a nodule recovered from the roots of the annualMedicago polymorpha L. growing in alkaline soil (pH 8.0) in Tel Afer, Iraq. WSM244 is the only acid-sensitiveE. medicae strain that has been sequenced to date. It is effective at fixing nitrogen withM. polymorpha L., as well as with more alkaline-adaptedMedicago spp. such asM. littoralis Loisel., M. scutellata (L.) Mill., M. tornata (L.) Mill. andM. truncatula Gaertn. This strain is also effective with the perennialM. sativa L. Here we describe the features ofE. medicae WSM244, together with genome sequence information and its annotation. The 6, 650, 282 bp high-quality permanent draft genome is arranged into 91 scaffolds of 91 contigs containing 6, 427 protein-coding genes and 68 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal.