Complete genome sequence of Novosphingobium pentaromativorans US6-1T. Issue 1 (December 2015)

• Record Type: Journal Article
• Authors: Choi, Dong; Kwon, Yong; Kwon, Kae; Kim, Sang-Jin
• Is Part Of: Standards in genomic sciences. Volume 10:Issue 1(2015)
• Page Start: 1
• Page End: 8
• Publication Date: 2015-12
• Subjects: Polycyclic aromatic hydrocarbon -- Novosphingobium -- Megaplasmids -- Extradiol dioxygenase; Genomics -- Periodicals; Genomics -- Periodicals; Genomics; Periodicals; 572.8605
• DOI: 10.1186/s40793-015-0102-1
• Languages: English
• ISSNs: 1944-3277

Abstract

Abstract Novosphingobium pentaromativorans US6-1T is a species in the familySphingomonadaceae . According to the phylogenetic analysis based on 16S rRNA gene sequence of theN. pentaromativorans US6-1T and nine genome-sequenced strains in the genusNovosphingobium, the similarity ranged from 93.9 to 99.9 % and the highest similarity was found withNovosphingobium sp. PP1Y (99.9 %), whereas the ANI value based on genomes ranged from 70.9 to 93 % and the highest value was 93 %. This microorganism was isolated from muddy coastal bay sediments where the environment is heavily polluted by polycyclic aromatic hydrocarbons (PAHs). It was previously shown to be capable of degrading multiple PAHs, including benzo[a]pyrene. To further understand the PAH biodegradation pathways the previous draft genome of this microorganism was revised to obtain a complete genome using Illumina MiSeq and PacBio platform. The genome of strain US6-1T consists of 5, 457, 578 bp, which includes the 3, 979, 506 bp chromosome and five megaplasmids. It comprises 5110 protein-coding genes and 82 RNA genes. Here, we provide an analysis of the complete genome sequence which enables the identification of new characteristics of this strain.