A MEMS-based measurement system for evaluating the force-length relationship of human induced pluripotent stem cell-derived cardiomyocytes adhered on a substrate. (19th March 2019)

• Record Type: Journal Article
• Authors: Matsudaira, Kenei; Takahashi, Hidetoshi; Hirayama-Shoji, Kayoko; Nguyen, Thanh-Vinh; Tsukagoshi, Takuya; Shimoyama, Isao
• Is Part Of: Journal of micromechanics and microengineering. Volume 29:Number 5(2019:May)
• Publication Date: 2019-03-19
• Subjects: MEMS -- piezoresistive cantilever -- iPS-cell derived cardiomyocytes -- contractile force -- force-length relationship; Microelectromechanical systems -- Periodicals; Micromechanics -- Periodicals; 621.38105
• DOI: 10.1088/1361-6439/ab093d
• Languages: English
• ISSNs: 0960-1317

Abstract

Abstract: This paper reports on a method for evaluating the force-length relationship of adhering human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) on the substrate using a measurement system comprising of a micromachined movable plate and a piezoresistive force probe. The cells on the plate are stretched by pushing the movable plate with the piezoresistive cantilever, which is actuated by a piezo stage. The twitch forces and the applied stretch are measured quantitatively with the piezoresistive cantilever. The results demonstrated that the twitch forces of the hiPSC-CMs increased when a stretch was applied. This evaluation method improves the understanding of the intrinsic force-length relationship of hiPSC-CMs at the cellular scale.