Non-typhoidal Salmonella serotypes, antimicrobial resistance and co-infection with parasites among patients with diarrhea and other gastrointestinal complaints in Addis Ababa, Ethiopia. Issue 1 (December 2015)

• Record Type: Journal Article
• Authors: Eguale, Tadesse; Gebreyes, Wondwossen; Asrat, Daniel; Alemayehu, Haile; Gunn, John; Engidawork, Ephrem
• Is Part Of: BMC infectious diseases. Volume 15:Issue 1(2015)
• Page Start: 1
• Page End: 9
• Publication Date: 2015-12
• Subjects: Non-typhoidal Salmonella -- Antimicrobial resistance -- Prevalence -- Serotype; Communicable diseases -- Periodicals; Sexually Transmitted Diseases -- Periodicals; 616.905
• DOI: 10.1186/s12879-015-1235-y
• Languages: English
• ISSNs: 1471-2334

Abstract

Abstract Background Non-typhoidalSalmonella (NTS) is an important public health problem worldwide. Consumption of animal-derived food products and direct and/or indirect contact with animals are the major routes of acquiring infection with NTS. Published information, particularly on the serotype distribution of NTS among human patients with gastroenteritis and associated risk factors, is scarce in Ethiopia. This study investigated the prevalence, risk factors, serotype distribution and antimicrobial susceptibility ofSalmonella species among diarrheic out-patients attending health centers in Addis Ababa and patients with various gastrointestinal complaints at Tikur Anbessa Specialized Hospital (TASH). Methods Stool samples were cultured forSalmonella species according to the WHO Global Foodborne Infections Network laboratory protocol.Salmonella serotyping was conducted using slide agglutination and microplate agglutination techniques. Antibiotic susceptibility testing was performed using the disk diffusion method according to Clinical and Laboratory Standards Institute guidelines. Results A total of 59 (6.2 %) stool samples, out of 957 were culture positive forSalmonella species. Fifty-five (7.2 %) of 765 diarrheic patients from health centers and 4 (2.1 %) of 192 patients from TASH were culture positive forSalmonella species. Multivariable logistic regression analysis after adjusting for all other variables revealed statistically significant association ofSalmonella infection with consumption of raw vegetables (OR = 1.91, 95 % CI = 1.29–2.83, χ2 = 4.74, p = 0.025) and symptom of watery diarrhea (OR = 3.3, 95 % CI = 1.23–8.88, χ2 = 10.54, p = 0.005). Eleven serotypes were detected, and the most prominent wereS. Typhimurium (37.3 %), S . Virchow (34 %), andS. Kottbus (10.2 %). Other serotypes wereS. Miami, S. Kentucky, S . Newport, S . Enteritidis, S . Braenderup, S . Saintpaul, S . Concord andS. V:ROUGH-O. Resistance to three or more antimicrobials was detected in 27 (40.3 %) of the isolates. Resistance to five or more antimicrobials was detected in 17 (25.4 %). Resistance to individual antimicrobials was found at varying proportions: streptomycin (50; 74.6 %), nitrofurantoin (27; 40.3 %), sulfisoxazole (26; 38.8 %), kanamycin (23; 34.3 %), cephalothin (12; 17.9 %), and ampicillin (11; 16.4 %) respectively. TwoS . Kentucky, oneS . Typhimurium and oneS. Concord isolates were multi-drug resistant to more than 10 antimicrobials. Conclusions The study demonstrated significant association ofSalmonella infection with consumption of raw vegetables. There was no significant association ofSalmonella infection with co-occurring parasites. The study also showed the dominance ofS. Typhimurium andS . Virchow in primary health care units. Overall, prevalence of MDR was low compared to previous studies. Although their proportion was low, S. Kentucky andS. Concord demonstrated wider spectrum of MDR. Continuous monitoring of circulating serotypes, antimicrobial resistance profile and characterization on molecular resistance determinants is essential for proper treatment of patients and for identifying potential environmental origins of antimicrobial resistance.