Spatiotemporally Controlled Reorganization of Signaling Complexes in the Plasma Membrane of Living Cells. Issue 44 (30th September 2015)

• Record Type: Journal Article
• Authors: Wedeking, Tim; Löchte, Sara; Birkholz, Oliver; Wallenstein, Alexander; Trahe, Julia; Klingauf, Jürgen; Piehler, Jacob; You, Changjiang
• Is Part Of: Small. Volume 11:Issue 44(2015)
• Page Start: 5912
• Page End: 5918
• Publication Date: 2015-09-30
• Subjects: cell micropatterning -- cytokine signaling -- fluorescence imaging -- microcontact printing -- imaging, plasma membranes, type I interferon receptors; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201502132
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract : Triggered immobilization of proteins in the plasma membrane of living cells into functional micropatterns is established by using an adaptor protein, which is comprised of an antiGFP nanobody fused to the HaloTag protein. Efficient in situ reorganization of the type I interferon receptor subunits as well as intact, fully functional signaling complexes in living cells are achieved by this method.