Determination of bergenin based on the electrochemiluminescence quenching of tris(2, 2′‐bipyridine)‐ruthenium(II). (25th March 2015)

• Record Type: Journal Article
• Authors: Zhu, Yinggui; Zou, Lili; Dong, Qin; Jiang, Derong
• Is Part Of: Luminescence. Volume 30:Number 8(2015:Dec.)
• Page Start: 1269
• Page End: 1273
• Publication Date: 2015-03-25
• Subjects: bergenin -- electrochemiluminescence (ECL) -- quenching; Luminescence -- Periodicals; Bioluminescence -- Periodicals; Chemiluminescence -- Periodicals; Luminescence -- Periodicals; 535.35
• DOI: 10.1002/bio.2891
• Languages: English
• ISSNs: 1522-7235

Abstract

Abstract: Quenching effects of bergenin, based on the electrochemiluminescence (ECL) of the tris(2, 2′‐bipyridyl)‐ruthenium(II) (Ru(bpy)3 2+ )/tri‐n‐propylamine (TPrA) system in aqueous solution, is been described. The quenching behavior can be observed with a 100‐fold excess of bergenin over Ru(bpy)3 2+ . In the presence of 0.1 m TPrA, the Stern–Volmer constant ( K SV ) of the ECL quenching is as high as 1.16 × 10 4 M −1 for bergenin. The logarithmic plot of the inhibited ECL versus logarithmic plot of the concentration of bergenin was linear over the range 3.0 × 10 −6 –1.0 × 10 −4 mol/L. The corresponding limit of detection was 6.0 × 10 −7 mol/L for bergenin (S/N = 3). In the mechanism of quenching it is believed that the competition of the active free radicals between Ru(bpy)3 2+ /TPrA and bergenin was the key factor for the ECL inhibition of the system. Photoluminescence, cyclic voltammetry, coupled with bulk electrolysis, supports the supposition mechanism of the Ru(bpy)3 2+ /TPrA–bergenin system. Copyright © 2015 John Wiley & Sons, Ltd.