Optimization of diatom Hasleao strearia cultivation in different mediums and nutrients. (February 2019)

• Record Type: Journal Article
• Authors: Arsad, S; Stavrakakis, C; Turpin, V; Rossa, P; Risjani, Y; Sari, L A; Prasetiya, F S; Mouget, J-L
• Is Part Of: IOP conference series. Volume 236(2019)
• Publication Date: 2019-02
• Subjects: Earth sciences -- Periodicals; Environmental sciences -- Congresses; Environmental sciences -- Periodicals; 550.5
• DOI: 10.1088/1755-1315/236/1/012044
• Languages: English
• ISSNs: 1755-1307

Abstract

Abstract: Hasleaostrearia is a marine diatom that can feed oysters. This study aimed to cultivate H.ostrearia in the following media: seawater (SW), seawater filtered by activated carbon (SW+CA), and drilling water (DW). The three-water media were supplemented with two types of nutrients, Walne-Conway+silica (CS) and ES-1/3 Provasoli (P), leading to a six experiment series. The cultures were grown in 200 mL and 400 mL respectively. The dilution cycle was every 3 - 4 day for 200 mL volume and every 4 - 5 days for the 400 mL volume for 20 - 40 days or until the cultures showed a decrease in growth. The microalgae exhibited a better growth in SW and SW+CA CS, whereas DW showed a diminishing number of cells in both the CS and P medium. The highest value of Emn in the 200 mL volume presented in the SW CS medium (1.94 mg L −1 ) and in the SW+CA CS medium (8.16 mg L −1 ), while the lowest value of Emn was found in the SW+CA CS medium (0.42 mg L −1 ). Imn was apparent in the SW P medium (0.56 mg L −1 ). In conclusion, both SW CS and SW+CA CS showed a better performance in growth. The seawater was more stable during the experiment in both the 200 mL and 400 mL volumes.