Antimicrobial activity of mul-1867, a novel antimicrobial compound, against multidrug-resistant Pseudomonas aeruginosa. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Tetz, George; Vikina, Daria; Tetz, Victor
• Is Part Of: Annals of clinical microbiology and antimicrobials. Volume 15:Issue 1(2016)
• Page Start: 1
• Page End: 6
• Publication Date: 2016-12
• Subjects: Biofilms -- Mucoid -- Non-mucoid -- Multidrug-resistant -- P. aeruginosa -- Cystic fibrosis; Medical microbiology -- Periodicals; Anti-infective agents -- Periodicals; 616.9041
• DOI: 10.1186/s12941-016-0134-4
• Languages: English
• ISSNs: 1476-0711

Abstract

Abstract Background There is an urgent need for new antimicrobial compounds to treat various lung infections caused by multidrug-resistantPseudomonas aeruginosa (MDR-PA). Methods We studied the potency of Mul-1867 against MDR-PA isolates from patients with cystic fibrosis, chronic obstructive pulmonary disease, and ventilator-associated pneumonia. The minimal inhibitory concentrations (MICs) and minimum biofilm eliminating concentrations (MBECs), defined as the concentrations of drug that kill 50 % (MBEC50 ), 90 % (MBEC90 ), and 100 % (MBEC100 ) of the bacteria in preformed biofilms, were determined by using the broth macrodilution method. Results Mul-1867 exhibited significant activity against MDR-PA and susceptible control strains, with MICs ranging from 1.0 to 8.0 µg/mL. Mul-1867 also possesses anti-biofilm activity against mucoid and non-mucoid 24-h-old MDR-PA biofilms. The MBEC50 value was equal to onefold the MIC. The MBEC90 value ranged from two to fourfold the MIC. Moreover, Mul-1867 completely eradicated mature biofilms at the concentrations tested, with MBEC100 values ranging between 16- and 32-fold the MIC. Mul-1867 was non-toxic to Madin-Darby canine kidney (MDCK) cells at concentrations up to 256 µg/mL. Conclusion Overall, these data indicate that Mul-1867 is a promising locally acting antimicrobial for the treatment and prevention ofP. aeruginosa infections.