Single wall and multiwall carbon nanotubes induce different toxicological responses in rat alveolar macrophages. Issue 5 (3rd January 2019)

• Record Type: Journal Article
• Authors: Nahle, Sara; Safar, Ramia; Grandemange, Stéphanie; Foliguet, Bernard; Lovera‐Leroux, Mélanie; Doumandji, Zahra; Le Faou, Alain; Joubert, Olivier; Rihn, Bertrand; Ferrari, Luc
• Is Part Of: Journal of applied toxicology. Volume 39:Issue 5(2019)
• Page Start: 764
• Page End: 772
• Publication Date: 2019-01-03
• Subjects: apoptosis -- in vitro -- inflammation -- multiwall carbon nanotubes -- MWCNT -- oxidative stress -- rat macrophages cell line NR8383 -- single wall carbon nanotubes -- SWCNT; Toxicology -- Periodicals; Industrial toxicology -- Periodicals; Environmentally induced diseases -- Periodicals; Toxicology -- Periodicals; 615.9005
• DOI: 10.1002/jat.3765
• Languages: English
• ISSNs: 0260-437X

Abstract

Abstract: Human exposure to airborne carbon nanotubes (CNT) is increasing because of their applications in different sectors; therefore, they constitute a biological hazard. Consequently, developing studies on CNT toxicity become a necessity. CNTs can have different properties in term of length, size and charge. Here, we compared the cellular effect of multiwall (MWCNTs) and single wall CNTs (SWCNTs). MWCNTs consist of multiple layers of graphene, while SWCNTs are monolayers. The effects of MWCNTs and SWCNTs were evaluated by the water‐soluble tetrazolium salt cell proliferation assay on NR8383 cells, rat alveolar macrophage cell line (NR8383). After 24 hours of exposure, MWCNTs showed higher toxicity (50% inhibitory concentration [IC50 ] = 3.2 cm 2 /cm 2 ) than SWCNTs (IC50 = 44 cm 2 /cm 2 ). Only SWCNTs have induced NR8383 cells apoptosis as assayed by flow cytometry using the annexin V/IP staining test. The expression of genes involved in oxidative burst ( Ncf1 ), inflammation ( Nfκb, Tnf‐α, Il‐6 and Il‐1β ), mitochondrial damage ( Opa ) and apoptotic balance ( Pdcd4, Bcl‐2 and Casp‐8 ) was determined. We found that MWCNT exposure predominantly induce inflammation, while SWCNTs induce apoptosis and impaired mitochondrial function. Our results clearly suggest that MWCNTs are ideal candidates for acute inflammation induction. In vivo studies are required to confirm this hypothesis. However, we conclude that toxicity of CNTs is dependent on their physical and chemical characteristics. Abstract : The physical and chemical properties of carbon nanotubes (CNTs) are of interest in many fields, particularly in the biomedical one. Consequently, human exposure to different CNTs is growing. Therefore, using NR8383 cells, rat alveolar macrophages, we evaluated modifications in gene expression after exposure to multiwall CNTs (NM403) and single wall CNTs (NRCWE‐055). We found different NR8383 cell responses, which confirm that CNT toxicity depends on physical and chemical characteristics of CNTs.