Enhanced production of polyhydroxybutyrate by multiple dividing E. coli. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Wu, Hong; Fan, Zhongyun; Jiang, Xiaoran; Chen, Jinchun; Chen, Guo-Qiang
• Is Part Of: Microbial cell factories. Volume 15:Issue 1(2016)
• Page Start: 1
• Page End: 13
• Publication Date: 2016-12
• Subjects: Cell fission -- Growth pattern -- PHB -- Escherichia coli -- Cell morphology -- Inclusion body -- Synthetic biology; Microbial biotechnology -- Periodicals; Recombinant proteins -- Synthesis -- Periodicals; 660.62
• DOI: 10.1186/s12934-016-0531-6
• Languages: English
• ISSNs: 1475-2859

Abstract

Abstract Background Most bacteria are grown in a binary fission way meaning a bacterial cell is equally divided into two. Polyhydroxyalkanoates (PHA) can be accumulated as inclusion bodies by bacteria. The cell division way and morphology have been shown to play an important role in regulating the bacterial growth and PHA storages. Results The common growth pattern ofEscherichia coli was changed to multiple fission patterns by deleting fission related genesminC andminD together, allowing the formation of multiple fission rings (Z-rings) in several positions of an elongated cell, thus a bacterial cell was observed to be divided into more than two daughter cells at same time. To further improve cell growth and PHA production, some genes related with division process includingftsQ, ftsL, ftsW, ftsN andftsZ, together with the cell shape control genemreB, were all overexpressed inE. coli JM109∆minCD . The changing pattern ofE. coli cell growth and morphology resulted in more cell dry weights (CDW) and more than 80 % polyhydroxybutyrate (PHB) accumulation increases compared to its binary fission control grown under the same conditions. Conclusions This study clearly demonstrated that combined over-expression genesftsQ, ftsW, ftsN, ftsL andftsZ together with shape control genemreB in multiple division bacterialE. coli JM109∆minCD benefited PHA accumulation. Our study provides useful information on increasing the yield of PHA by changing the cell division pattern and cell morphology ofE. coli .