Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology. Issue 1 (December 2015)

• Record Type: Journal Article
• Authors: Gasser, Brigitte; Steiger, Matthias; Mattanovich, Diethard
• Is Part Of: Microbial cell factories. Volume 14:Issue 1(2015)
• Page Start: 1
• Page End: 3
• Publication Date: 2015-12
• Subjects: Pichia pastoris -- Komagataella -- Promoter -- Induction -- Synthetic biology -- Protein production; Microbial biotechnology -- Periodicals; Recombinant proteins -- Synthesis -- Periodicals; 660.62
• DOI: 10.1186/s12934-015-0387-1
• Languages: English
• ISSNs: 1475-2859

Abstract

Abstract Promoters are indispensable elements of a standardized parts collection for synthetic biology. Regulated promoters of a wide variety of well-defined induction ratios and expression strengths are highly interesting for many applications. Exemplarily, we discuss the application of published genome scale transcriptomics data for the primary selection of methanol inducible promoters of the yeastPichia pastoris (Komagataella sp.). Such a promoter collection can serve as an excellent toolbox for cell and metabolic engineering, and for gene expression to produce heterologous proteins.