The impact and origin of copy number variations in the Oryza species. (December 2016)

• Record Type: Journal Article
• Authors: Bai, Zetao; Chen, Jinfeng; Liao, Yi; Wang, Meijiao; Liu, Rong; Ge, Song; Wing, Rod; Chen, Mingsheng
• Is Part Of: BMC genomics. Volume 17:Number 1(2016)
• Page Start: 1
• Page End: 12
• Publication Date: 2016-12
• Subjects: Oryza species -- Copy number variation (CNV) -- NGS-based survey -- CNV genes -- Mutation mechanisms; Genomes -- Periodicals; Gene mapping -- Periodicals; Genomics -- Periodicals; Base Sequence -- Periodicals; Chromosome Mapping -- Periodicals; Genetic Techniques -- Periodicals; Sequence Analysis, DNA -- Periodicals; 572.8605
• DOI: 10.1186/s12864-016-2589-2
• Languages: English
• ISSNs: 1471-2164

Abstract

Abstract Background Copy number variation (CNV), a complex genomic rearrangement, has been extensively studied in humans and other organisms. In plants, CNVs of several genes were found to be responsible for various important traits; however, the cause and consequence of CNVs remains largely unknown. Recently released next-generation sequencing (NGS) data provide an opportunity for a genome-wide study of CNVs in rice. Results Here, by an NGS-based approach, we generated a CNV map comprising 9, 196 deletions compared to the reference genome 'Nipponbare'. UsingOryza glaberrima as the outgroup, 80 % of the CNV events turned out to be insertions in Nipponbare. There were 2, 806 annotated genes affected by these CNV events. We experimentally validated 28 functional CNV genes includingOsMADS56, BPH14, OsDCL2b andOsMADS30, implying that CNVs might have contributed to phenotypic variations in rice. Most CNV genes were found to be located in non-co-linear positions by comparison toO. glaberrima. One of the origins of these non-co-linear genes was genomic duplications caused by transposon activity or double-strand break repair. Comprehensive analysis of mutation mechanisms suggested an abundance of CNVs formed by non-homologous end-joining and mobile element insertion. Conclusions This study showed the impact and origin of copy number variations in rice on a genomic scale.