Genome-wide identification and evolutionary analyses of bZIP transcription factors in wheat and its relatives and expression profiles of anther development related TabZIP genes. (December 2015)

• Record Type: Journal Article
• Authors: Li, Xueyin; Gao, Shiqing; Tang, Yimiao; Li, Lei; Zhang, Fengjie; Feng, Biane; Fang, Zhaofeng; Ma, Lingjian; Zhao, Changping
• Is Part Of: BMC genomics. Volume 16:Number 1(2015)
• Page Start: 1
• Page End: 21
• Publication Date: 2015-12
• Subjects: Basic leucine zipper (bZIP) -- Common wheat -- Orthology analyses -- Gene expression -- Anther development -- Over-dominance; Genomes -- Periodicals; Gene mapping -- Periodicals; Genomics -- Periodicals; Base Sequence -- Periodicals; Chromosome Mapping -- Periodicals; Genetic Techniques -- Periodicals; Sequence Analysis, DNA -- Periodicals; 572.8605
• DOI: 10.1186/s12864-015-2196-7
• Languages: English
• ISSNs: 1471-2164

Abstract

Abstract Background Among the largest and most diverse transcription factor families in plants, basic leucine zipper (bZIP) family participate in regulating various processes, including floral induction and development, stress and hormone signaling, photomorphogenesis, seed maturation and germination, and pathogen defense. Although common wheat (Triticum aestivum L.) is one of the most widely cultivated and consumed food crops in the world, there is no comprehensive analysis of bZIPs in wheat, especially those involved in anther development. Previous studies have demonstrated wheat, T. urartu, Ae. tauschii, barley andBrachypodium are evolutionarily close in Gramineae family, however, the real evolutionary relationship still remains mysterious. Results In this study, 187 bZIP family genes were comprehensively identified from current wheat genome. 98, 96 and 107 members of bZIP family were also identified from the genomes ofT.urartu, Ae.tauschii and barley, respectively. Orthology analyses suggested 69.4 % of TubZIPs were orthologous to 68.8 % of AetbZIPs and wheat had many more in-paralogs in the bZIP family than its relatives. It was deduced wheat had a closer phylogenetic relationship with barley andBrachypodium thanT.urartu andAe.tauschii . bZIP proteins in wheat, T.urartu andAe.tauschii were divided into 14 subgroups based on phylogenetic analyses. Using Affymetrix microarray data, 48 differentially expressedTabZIP genes were identified to be related to anther development from comparison between the male sterility line and the restorer line. Genes with close evolutionary relationship tended to share similar gene structures. 15 of 23 selectedTabZIP genes contained LTR elements in their promoter regions. Expression of 21 among these 23TabZIP genes were obviously responsive to low temperature. These 23TabZIP genes all exhibited distinct tissue-specific expression pattern. Among them, 11TabZIP genes were predominantly expressed in anther and most of them showed over-dominance expression mode in the cross combination TY806 × BS366. Conclusions The genome-wide identification provided an overall insight ofbZIP gene family in wheat and its relatives. The evolutionary relationship of wheat and its relatives was proposed based on orthology analyses. Microarray and expression analyses suggested the potential involvement ofbZIP genes in anther development and facilitated selection of anther development related gene for further functional characterization.