Genome-wide identification and characterization of the superoxide dismutase gene family in Musa acuminata cv. Tianbaojiao (AAA group). (December 2015)

• Record Type: Journal Article
• Authors: Feng, Xin; Lai, Zhongxiong; Lin, Yuling; Lai, Gongti; Lian, Conglong
• Is Part Of: BMC genomics. Volume 16:Number 1(2015)
• Page Start: 1
• Page End: 16
• Publication Date: 2015-12
• Subjects: Musa acuminata cv. Tianbaojiao (AAA group) -- SOD gene family -- Promoter -- Abiotic stress -- Hormonal stress -- Expression analysis; Genomes -- Periodicals; Gene mapping -- Periodicals; Genomics -- Periodicals; Base Sequence -- Periodicals; Chromosome Mapping -- Periodicals; Genetic Techniques -- Periodicals; Sequence Analysis, DNA -- Periodicals; 572.8605
• DOI: 10.1186/s12864-015-2046-7
• Languages: English
• ISSNs: 1471-2164

Abstract

Abstract Background Superoxide dismutase (SOD) is an essential enzyme of the plant antioxidant system that responds to oxidative stresses caused by adverse conditions. Banana is an important staple and economic crop in tropical and subtropical regions. However, its growth and yield are constantly affected by various abiotic stresses. To analyze the roles of distinctSOD genes under various stresses, a detailed characterization and analysis of theSOD gene family in Cavendish banana is indispensable. Methods The presence and structure of the SOD family genes were experimentally verified using 5′/3′ RACE-PCR, reverse transcription PCR and PCR. Then, their syntenic relationships, conserved motifs and phylogenetic relationships were analyzed using software. Cis-elements present in the promoters were predicted via PlantCARE. And the expression levels under abiotic and hormonal stresses were determined using real-time quantitative polymerase chain reaction. Results In total, 25 'Tianbaojiao'SOD cDNAs (MaSODs ), which encoded six Cu/ZnSODs, four MnSODs and two FeSODs, were cloned. The 12MaSOD genes were divided into four groups based on their conserved motifs, which corroborated their classifications based on gene-structure patterns and subcellular localizations. ElevenMaSOD promoters were isolated and found to contain manycis -acting elements involved in stress responses. Gene expression analysis showed that 11 out of the 12MaSOD s were expressed in all tested tissues (leaf, pseudostem and root), whereasMaCSD2B was expressed only in leaves and roots. SpecificMaSOD members exhibited different expression patterns under abiotic and hormonal treatments. Among the 12MaSOD genes, MaCSD1D was the only one that responded to all eight treatments, suggesting that this gene plays a predominant role in reactive oxygen species scavenging caused by various stresses in banana. Conclusions A genome-wide analysis showed that the 'Tianbaojiao' banana harbored an expandedSOD gene family. Whole genome duplication, segmental duplication and complex transcriptional regulation contributed to the gene expansion and mRNA diversity of theMaSODs . The expression patterns of distinctMaSOD genes showed that they are important responses to different abiotic and hormonal stresses in banana.