Characterizing human lung tissue microbiota and its relationship to epidemiological and clinical features. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Yu, Guoqin; Gail, Mitchell; Consonni, Dario; Carugno, Michele; Humphrys, Michael; Pesatori, Angela; Caporaso, Neil; Goedert, James; Ravel, Jacques; Landi, Maria
• Is Part Of: Genome biology. Volume 17:Issue 1(2016)
• Page Start: 1
• Page End: 12
• Publication Date: 2016-12
• Subjects: Air pollution -- Tumor stage -- 16S rRNA; Genomes -- Periodicals; Biology -- Periodicals; Molecular biology -- Periodicals; 572.8633
• DOI: 10.1186/s13059-016-1021-1
• Languages: English
• ISSNs: 1474-760X

Abstract

Abstract Background The human lung tissue microbiota remains largely uncharacterized, although a number of studies based on airway samples suggest the existence of a viable human lung microbiota. Here we characterized the taxonomic and derived functional profiles of lung microbiota in 165 non-malignant lung tissue samples from cancer patients. Results We show that the lung microbiota is distinct from the microbial communities in oral, nasal, stool, skin, and vagina, withProteobacteria as the dominant phylum (60 %). Microbiota taxonomic alpha diversity increases with environmental exposures, such as air particulates, residence in low to high population density areas, and pack-years of tobacco smoking and decreases in subjects with history of chronic bronchitis. GenusThermus is more abundant in tissue from advanced stage (IIIB, IV) patients, whileLegionella is higher in patients who develop metastases. Moreover, the non-malignant lung tissues have higher microbiota alpha diversity than the paired tumors. Conclusions Our results provide insights into the human lung microbiota composition and function and their link to human lifestyle and clinical outcomes. Studies among subjects without lung cancer are needed to confirm our findings.