MRNA/lncRNA expression patterns and the function of fibrinogen‐like protein 2 in Meishan pig endometrium during the preimplantation phases. Issue 4 (7th February 2019)

• Record Type: Journal Article
• Authors: Wang, Yueying; Hua, Renwu; Xue, Songyi; Li, Wenchao; Wu, Lihang; Kang, Tingting; Lei, Minggang
• Is Part Of: Molecular reproduction and development. Volume 86:Issue 4(2019)
• Page Start: 354
• Page End: 369
• Publication Date: 2019-02-07
• Subjects: endometrium -- FGL2 -- gene expression -- implantation -- pigs; Reproduction -- Periodicals; Molecular biology -- Periodicals; Molecular genetics -- Periodicals; Embryology -- Periodicals; 571.8
• DOI: 10.1002/mrd.23109
• Languages: English
• ISSNs: 1040-452X

Abstract

Abstract : Embryonic implantation involves a complex and well‐coordinated interaction between the developing conceptus and maternal uterus, and the preimplantation period has a major impact on litter size in pigs. The present study aimed to investigate the vital messenger RNAs (mRNAs) and long noncoding RNAs (lncRNAs) that regulate preimplantation in Meishan pigs. The enriched Gene Ontology terms were all related to "binding." Furthermore, "ECM‐receptor interaction" was predicted as an important pathway that regulated the success of implantation. We speculated that the differentially expressed mRNAs S100A9, ANXA8, MUC16, and FGL2 and the differentially expressed lncRNAs TCONS_11206566, TCONS_09904861, and TCONS_1252933 may play vital roles in the process of implantation. Furthermore, this study verified that FGL2 was highly expressed on Day 12 of pregnancy, and we also investigated the function of FGL2 during preimplantation in vivo. In conclusion, this study provides useful information for further analyses of the molecular mechanisms of implantation in Chinese domestic pigs. Abstract : We identified the expression patterns of messenger RNAs and long noncoding RNAs based on the paired‐end RNA‐seq data obtained from the endometrial samples of pigs. Furthermore, we identified that FGL2 was highly expressed on Day 12 of pregnancy, and it plays an important role in embryo implantation.