A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury. Issue 8 (18th March 2019) - Xu, Runfeng
Abstract : A fluorescent probe, Cy-ArB, is developed for real-time monitoring of H2 O2 fluctuations in cells and in vivo during ischemia/reperfusion processes. Abstract : Hydrogen peroxide (H2 O2 ), as a major component of reactive oxygen species (ROS), plays an important role in normal physiological processes. A H2 O2 burst also occurs in the ischemia/reperfusion (I/R) process and causes a series of physiological and pathological injuries. Therefore, it is important to determine concentration fluctuations of H2 O2 . Here we develop a ratiometric fluorescent probe, Cy-ArB, which shows high selectivity and sensitivity toward H2 O2 . The fluorescence response of the probe is triggered by the reaction of borate esters with H2 O2, and this process releases a near-infrared heptamethine cyanine fluorophore which has the ability of mitochondrial tracing. Hence, the probe can be used for real-time monitoring of H2 O2 fluctuations in the mitochondrial respiration chain. Finally, we explore the fluctuations of H2 O2 in cells and in vivo during the I/R process using the probe Cy-ArB. The results of our experiments prove that our probe is a potential candidate for clinical surgery pre-evaluation.