Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction. Issue 8 (6th March 2019)

• Record Type: Journal Article
• Authors: Morimoto, Takeshi; Chiu, Liang-da; Kanda, Hiroyuki; Kawagoe, Hiroyuki; Ozawa, Takeaki; Nakamura, Makoto; Nishida, Kohji; Fujita, Katsumasa; Fujikado, Takashi
• Is Part Of: Analyst. Volume 144:Issue 8(2019)
• Page Start: 2531
• Page End: 2540
• Publication Date: 2019-03-06
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c8an02213e
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : We present a label-free imaging technique that detects mitochondrial activities with a sub-cellular spatial resolution. Abstract : Mitochondrial activity is a widely used criterion to judge the metabolic condition of a living specimen. Numerous methods have been developed for related analyses, including the detection of O2 consumption, trans-membrane potential, and ATP production. In this study, we demonstrate that the redox state of cytochromes can serve as a sensitive mitochondrial activity indicator in glutamate-stressed neuronal cells. Mitochondrial dysfunction was detected by Raman imaging as early as 30 min after glutamate-stress induction. By comparing this result with other commonly used mitochondrial function assays, we found Raman imaging has a similar sensitivity to ATP production and trans-membrane potential assays. Other viability tests, such as MTT assay and ROS production tests, showed a slower response than our method. A thorough understanding of cytochrome dynamics with our new method will help establish Raman spectroscopy as a competitive clinical diagnosis tool for neurodegenerative diseases involving mitochondrial dysfunction.