A novel fluorescent surfactant enhances the delivery of the Cas9 ribonucleoprotein and enables the identification of edited cells. Issue 31 (1st April 2019)
- Record Type:
- Journal Article
- Title:
- A novel fluorescent surfactant enhances the delivery of the Cas9 ribonucleoprotein and enables the identification of edited cells. Issue 31 (1st April 2019)
- Main Title:
- A novel fluorescent surfactant enhances the delivery of the Cas9 ribonucleoprotein and enables the identification of edited cells
- Authors:
- Li, Jie
Røise, Joachim Justad
Zhang, Jingtuo
Yang, Jiaying
Kerr, D. Lucas
Han, Hesong
Murthy, Niren - Abstract:
- Abstract : A novel fluorescent single tailed surfactant was synthesized, which can disrupt endosomes, complex lipofectamine, and identify transfected cells. Abstract : In this report, we designed and synthesized a novel fluorescent single tailed surfactant (termed FEDS), which can disrupt endosomes, complex lipofectamine, and can also identify cells that have been transfected. FEDS was able to increase the gene editing efficiency of lipofectamine/Cas9 ribonucleoprotein by 300% via a combination of fluorescent based enrichment and endosomal disruption.
- Is Part Of:
- Chemical communications. Volume 55:Issue 31(2019)
- Journal:
- Chemical communications
- Issue:
- Volume 55:Issue 31(2019)
- Issue Display:
- Volume 55, Issue 31 (2019)
- Year:
- 2019
- Volume:
- 55
- Issue:
- 31
- Issue Sort Value:
- 2019-0055-0031-0000
- Page Start:
- 4562
- Page End:
- 4565
- Publication Date:
- 2019-04-01
- Subjects:
- Chemistry -- Periodicals
540 - Journal URLs:
- http://pubs.rsc.org/en/journals/journalissues/cc ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/c9cc00261h ↗
- Languages:
- English
- ISSNs:
- 1359-7345
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 3139.350000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 9845.xml