Structural characterization and anti-inflammatory effect in hepatocytes of a galactoglucan from Antrodia camphorata mycelium. Issue 14 (8th March 2019)

• Record Type: Journal Article
• Authors: Tang, Huiling; Nie, Wenbing; Xiao, Jinna; Zha, Zhengqi; Chen, Qiuli; Yin, Hongping
• Is Part Of: RSC advances. Volume 9:Issue 14(2019)
• Page Start: 7664
• Page End: 7672
• Publication Date: 2019-03-08
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c8ra10347j
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A previously undescribed polysaccharide ACP2 was isolated from Antrodia camphorata mycelium. ACP2 ameliorated hepatocyte inflammation through TLR4 and p38/NF-κB signal pathway. Abstract : The galactoglucan ACP2 was isolated from cultured Antrodia camphorata mycelium through anion-exchange column chromatography and Sephadex G-100 chromatography and shown to exhibit hepatoprotective function in L02 cells. Based on monosaccharide composition analysis, ACP2 was mainly composed of glucose, galactose, and 6-deoxyglucose in a molar ratio of 5 : 2 : 1. The average molecular weight of ACP2 was 1.93 × 10 4 Da. The primary structure of ACP2 was elucidated with Fourier-transform infrared spectroscopy, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy. The results indicated the following composition: →6)-linked-β-d -Gal p -(1→, →6)-linked-α-d -Glc p -(1→, →3)-linked-α-d -Glc p -(1→, and →2, 4)-linked-β-d -Glc p -(1→, with terminal 6-deoxy-α-d -Glc p and α-d -Glc p . ACP2 alleviated lipopolysaccharide-induced hepatocyte inflammation by down-regulating the expressions of COX-2, IL-1β, TNF-α and IL-6. The decreased expressions of TLR4, MyD88, NF-κB, and phosphorylated p38 in ACP2-treated L02 cells indicated that ACP2 might ameliorate inflammation through the TLR4 and p38/NF-κB signaling pathways.