Hydrodynamic shuttling for deterministic high-efficiency multiple single-cell capture in a microfluidic chip. Issue 8 (19th March 2019)

• Record Type: Journal Article
• Authors: He, Cheng-Kun; Chen, Ya-Wen; Wang, Ssu-Han; Hsu, Chia-Hsien
• Is Part Of: Lab on a chip. Volume 19:Issue 8(2019)
• Page Start: 1370
• Page End: 1377
• Publication Date: 2019-03-19
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c9lc00036d
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : A new microfluidics technique for high-efficiency paring and analyzing multiple single cells can facilitate cellular heterogeneity studies important for biological and biomedical research. Abstract : Studies on cellular heterogeneity have emerged as a powerful approach for developing new strategies to treat diseases including cancer. However, it is difficult to set up an in vitro co-culture experiment to study the interaction of individual live cells. In this paper, we report a hydrodynamic shuttling chip (HSC) which can deterministically capture single cells into microfluidic chambers to set up multiple single-cell co-culture experiments in which individual live cells can be microscopically observed. Using this chip device, we demonstrated a triple single-cell culture of oral squamous cell carcinoma and lymphatic endothelial cells to observe the effect of cell–cell interaction on the cell motility. Triple, single-cell pairing efficiency by our HSC device was eightfold higher than that of the probabilistic method. Using this HSC device, we were able to perform triple-culture experiments to show the cell type-dependent motility of oral squamous cell carcinoma and lymphatic endothelial cells, which was not observed in co-culture experiments.