A miniaturized push–pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain. Issue 8 (14th March 2019)

• Record Type: Journal Article
• Authors: van den Brink, Floris T. G.; Phisonkunkasem, Thas; Asthana, Ashish; Bomer, Johan G.; van den Maagdenberg, Arn M. J. M.; Tolner, Else A.; Odijk, Mathieu
• Is Part Of: Lab on a chip. Volume 19:Issue 8(2019)
• Page Start: 1332
• Page End: 1343
• Publication Date: 2019-03-14
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c8lc01137k
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : Measuring neurotransmitters in the brain of living animals is a challenging task, especially when detailed information at high temporal resolution is required. Abstract : Measuring biomolecule concentrations in the brain of living animals, in real time, is a challenging task, especially when detailed information at high temporal resolution is also required. Traditionally, microdialysis probes are used that generally have sampling areas in the order of about 1 mm 2, and provide information on concentrations with a temporal resolution of at least several minutes. In this paper, we present a novel miniaturized push–pull perfusion sampling probe that uses an array of small 3 μm-wide sampling channels to sample neurotransmitters at a typical recovery rate of 61%, with a reduced risk of clogging. The added feature to segment the dialysate inside the probe into small water-in-decane droplets enables the detection of concentrations with a temporal resolution of a few seconds. Here we used the probe for in vivo recordings of neurotransmitter glutamate released upon electrical stimulation in the brain of a mouse to demonstrate the feasibility of the probe for real-time neurochemical brain analysis.