Structural characterization of centipede oligopeptides and capability detection in human small cell lung carcinoma: inducing apoptosis. Issue 19 (8th April 2019)

• Record Type: Journal Article
• Authors: Zhao, JingQuan; Yang, Jianmei; Hao, Zerui; An, Yulin; Zhang, Mingqiang; Liu, Jie; Ren, Rongrong; Lin, Dianjie
• Is Part Of: RSC advances. Volume 9:Issue 19(2019)
• Page Start: 10927
• Page End: 10936
• Publication Date: 2019-04-08
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c8ra09018a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : SSMO-5 mediated the lung cancer cells apoptosis by activating the caspases and regulating the interaction of p53/MDM2. Abstract : Lung cancer is the most frequent cause of cancer deaths in the world, and smoking is considered as one of the major causes. Small cell lung carcinoma (SCLC) represents a highly malignant and particularly aggressive form, with properties of widespread metastases and poor prognosis. Herein, twenty-five Scolopendra subspinipes mutilans L. Koch Oligopeptides (SSMOs) were isolated and their structures were identified, and the anti-proliferative activity against lung cancer cell lines was evaluated. Results showed that SSMO-5 induced the production of reactive oxygen species (ROS) markedly in NCI-H446 cells. Furthermore, SSMO-5 decreased the mitochondrial membrane potential (MMP) and enhanced the mitochondria-related apoptosis. These results demonstrate that in NCI-H446 cells, the apoptotic and cytotoxic effects of SSMO-5 are mediated by the intrinsic mitochondria-mediated apoptotic pathway, which in turn causes the activation of caspases and increases Bax expression, while decreases Bcl-2 and Bcl-xL expressions and regulates the interaction of p53/MDM2. In conclusion, a ROS-mediated mitochondrial pathway plays an important role in the process of SSMO-5-induced apoptosis against SCLC.