RNA-seq analysis of different inflammatory reactions induced by lipopolysaccharide and lipoteichoic acid in bovine mammary epithelial cells. (May 2019)

• Record Type: Journal Article
• Authors: Xu, Tong; Deng, Ruizhi; Li, Xuezhong; Zhang, Yong; Gao, Ming-Qing
• Is Part Of: Microbial pathogenesis. Volume 130(2019)
• Page Start: 169
• Page End: 177
• Publication Date: 2019-05
• Subjects: Bovine mammary epithelial cells -- Bovine mastitis -- Lipopolysaccharide -- Lipoteichoic acid -- RNA-Seq; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2019.03.015
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Lipopolysaccharide (LPS) and lipoteichoic acid (LTA) are key virulence factors of Escherichia coli and Staphylococcus aureus respectively, and both of them could cause inflammatory reaction in bovine mammary glands. In this study, we used bovine mammary epithelial cells (BMECs) as pattern recognition receptors and stimulated them with LPS or LTA to investigate the global transcriptional response variations of BMECs to these two different virulent factors through RNA-Seq analysis. We found 100 differentially expressed genes (DEGs) with 95 up-regulated and 5 down-regulated genes in LPS-treated group, whereas 24 DEGs with 12 up-regulated and 12 down-regulated genes in LTA-treated group compared to control. Although the number and expression changes of DEGs are significantly different between LPS vs Control and LTA vs Control, KEGG pathway enrichment analysis showed the majorities of DEGs in each pair were enriched on cytokine-cytokine receptor interaction, NF-κB signaling pathway, and NOD-like receptor signaling pathway, especially cytokines and chemokines. These results provided a comprehensive analysis of gene expression profiles elicited by LPS and LTA in BMECs, contributing to the understanding of early "pathogen-host" interactions during intramammary infections. Highlights: RNA-seq analysis of differently expressed genes in LPS and LTA-treated bovine mammary epithelial cells. LPS and LTA causes various expression of chemokines and cytokines through different signaling pathways. LPS and LTA induce diverse inflammatory responses during bovine mastitis.