Effect of the isoelectric point of pH-responsive lignin-based amphoteric surfactant on the enzymatic hydrolysis of lignocellulose. (July 2019)

• Record Type: Journal Article
• Authors: Zhan, Xuejuan; Cai, Cheng; Pang, Yuxia; Qin, Feiyang; Lou, Hongming; Huang, Jinhao; Qiu, Xueqing
• Is Part Of: Bioresource technology. Volume 283(2019)
• Page Start: 112
• Page End: 119
• Publication Date: 2019-07
• Subjects: Quaternary ammonium lignin carboxylate -- Isoelectric point -- Lignocellulose -- Enzymatic hydrolysis -- Cellulase; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.03.026
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Lignin-based surfactants LC and LCQ with various isoelectric points were synthesized. The effect and mechanism of the pI of LCQ on the enzymatic hydrolysis were studied. The SED of Eu-SPORL increased from 35.2% to 95.3% by adding LCQ-40. LCQ could be efficiently recovered for the excellent pH-response performance. Abstract: The isoelectric point (pI) of lignin-based surfactant is an important factor in the enhancement on the enzymatic hydrolysis of lignocellulose. In this work, lignin carboxylate (LC) and quaternary ammonium lignin carboxylates (LCQ-x, x%: the mass ratio of quaternizing agent to enzymatic hydrolysis lignin) with different isoelectric points were synthesized. LC or LCQ-x with pI significantly lower or higher than 4.8 reduced the non-productive adsorption of cellulase on lignin, but for the significant inhibitory effect on cellulase activity, their enhancements on the enzymatic hydrolysis of lignocellulose were not remarkable. However, LCQ-x with pI around 4.8 preserved the cellulase activity, and significantly reduced the non-productive adsorption of cellulase, therefore remarkably enhanced the enzymatic hydrolysis. 2 g/L LC, LCQ-40 (pI = 5.0) and LCQ-100 (pI = 9.2) increased the enzymatic digestibility of pretreated eucalyptus from 35.2% to 53.4%, 95.3% and 60.4% respectively. In addition, for the excellent pH-response performance, LCQ could be efficiently recovered after enzymatic saccharification.