First report on USA300 outbreak in a neonatal intensive care unit detected by polymerase chain reaction-based open reading frame typing in Japan. Issue 5 (May 2019)

• Record Type: Journal Article
• Authors: Uehara, Yuki; Mori, Miki; Tauchi, Mutsuhiro; Nishimura, Shu; Sakurai, Hiroki; Murai, Takemi; Okazaki, Kaoru; Kinoshita, Kazue; Horikoshi, Yuho; Hiramatsu, Keiichi
• Is Part Of: Journal of infection and chemotherapy. Volume 25:Issue 5(2019:May)
• Page Start: 400
• Page End: 403
• Publication Date: 2019-05
• Subjects: Methicillin-resistant Staphylococcus aureus -- USA300 -- Neonatal intensive care unit -- Polymerase chain reaction-based open reading frame typing; Chemotherapy -- Periodicals; Infection -- Periodicals; Communicable diseases -- Chemotherapy -- Periodicals; 615.5805
• DOI: 10.1016/j.jiac.2018.12.002
• Languages: English
• ISSNs: 1341-321X

Abstract

Abstract: Outbreaks of methicillin-resistant Staphylococcus aureus (MRSA) in the neonatal intensive care unit (NICU) have been reported worldwide. Some outbreaks were caused by USA300, which is a community-associated MRSA clone. In 2011, polymerase chain reaction-based open reading frame typing (POT) for the initial MRSA isolates from all inpatients was started at the Tokyo Metropolitan Children's Medical Center. From March 2014 to April 2015, a total of 131 MRSA strains were isolated, 104 of which were analyzed as healthcare-associated MRSA. Thirteen stains (12.5%) had a POT number of 106-9-93, which strongly suggested USA300; these included 6 from nasal swabs, 6 from blood cultures and 1 from subcutaneous pus. All the MRSA strains were isolated from patients in the NICU; were typed as sequence type 8, spa type t008, and staphylococcal cassette chromosome type mec IVa; and possessed the lukS-lukF and arginine catabolic mobile element -arcA gene. Pulsed-field gel electrophoresis of all the strains, with USA300-0114 as a reference, showed indistinguishable banding pattern. Based on these results, POT was useful in recognizing this first MRSA outbreak of USA300 in a Japanese NICU and was advantageous in terms of swiftness, less cost and monitoring change of the epidemic MRSA lineage.