The presence of osteocalcin, osteopontin and reactive oxygen species‐positive cells in pulp tissue after dental bleaching. (18th December 2018)

• Record Type: Journal Article
• Authors: Benetti, F.; Briso, A. L. F.; Carminatti, M.; de Araújo Lopes, J. M.; Barbosa, J. G.; Ervolino, E.; Gomes‐Filho, J. E.; Cintra, L. T. A.
• Is Part Of: International endontic journal. Volume 52:Number 5(2019)
• Page Start: 665
• Page End: 675
• Publication Date: 2018-12-18
• Subjects: dental pulp -- hydrogen peroxide -- osteocalcin -- osteopontin -- reactive oxygen species -- tertiary dentine; Endodontics -- Periodicals; 617.6342
• DOI: 10.1111/iej.13049
• Languages: English
• ISSNs: 0143-2885

Abstract

Abstract: Aim: To analyse the influence of H2 O2 on pulp repair through osteocalcin and osteopontin immunolabelling and in cellular defence by using the antireactive oxygen species (ROS) antibody. Methodology: The maxillary molars of 50 rats were treated with 35% H2 O2 (Ble groups) or placebo gel (control groups). At 0 h and 2, 7, 15 and 30 days ( n = 10 hemimaxillae), the rats were killed and pulp tissue was evaluated using inflammation and immunolabelling scores (osteocalcin/osteopontin); ROS‐positive cells were counted. Paired t ‐test and Wilcoxon signed‐rank test were used ( P < 0.05). Results: The Ble group had necrosis in the coronal pulp at 0 h and in the occlusal third of the coronal pulp at 2 days; at 7, 15 and 30 days, no inflammation was noted similar to the controls ( P > 0.05). Osteocalcin was absent in the Ble at 0 h, moderate at 2 days and increased thereafter, differing from the controls at all two periods ( P < 0.05). Osteopontin was higher principally at 7 and 15 days in Ble groups, but differing with control groups from 2 days after bleaching ( P < 0.05). The Ble group had more ROS‐positive cells in the pulp at 7 and 15 days ( P < 0.05). Tertiary dentine was observed at 7 days, increasing thereafter ( P < 0.05). Conclusions: Post‐bleaching pulp repair was associated with increased osteocalcin over time. Osteopontin also participated in this process, and anti‐ROS was involved in cellular defence against H2 O2 .