Isolation and detection of microRNA from the egg of chickens. (December 2016)

• Record Type: Journal Article
• Authors: Wade, Ben; Cummins, Michelle; Keyburn, Anthony; Crowley, Tamsyn
• Is Part Of: BMC research notes. Volume 9:Number 1(2016)
• Page Start: 1
• Page End: 6
• Publication Date: 2016-12
• Subjects: microRNA -- Chicken -- Egg -- Yolk -- Albumen -- Extraction; Medicine -- Periodicals; Biology -- Periodicals; 610.5
• DOI: 10.1186/s13104-016-2084-5
• Languages: English
• ISSNs: 1756-0500

Abstract

Abstract Background The egg is a vital part of the chicken developmental process and an important protein source for humans. Despite the chicken egg being a subject of intense research little attention has been given to the role of microRNAs within the egg. Findings We report a method for the reproducible and reliable isolation of miRNA from the albumen and yolk of chicken eggs. We also report the detection via real-time PCR of a number of miRNAs from both of these biological fluids. Conclusions These findings provide an interesting look into the chicken egg and raise questions as to the role that miRNAs maybe playing in the chicken egg. This method of detecting miRNAs in chicken eggs will allow researchers to investigate the presence of an additional level of epigenetic programming in chick development previously unknown and also how this impacts the nutritional value of eggs for human consumption.