DNA methylation changes facilitated evolution of genes derived from Mutator-like transposable elements. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Wang, Jun; Yu, Yeisoo; Tao, Feng; Zhang, Jianwei; Copetti, Dario; Kudrna, Dave; Talag, Jayson; Lee, Seunghee; Wing, Rod; Fan, Chuanzhu
• Is Part Of: Genome biology. Volume 17:Issue 1(2016)
• Page Start: 1
• Page End: 20
• Publication Date: 2016-12
• Subjects: Comparative genomics -- DNA methylation -- GC content -- Molecular evolution -- MULEs -- New genes -- Oryza -- Recombination rate; Genomes -- Periodicals; Biology -- Periodicals; Molecular biology -- Periodicals; 572.8633
• DOI: 10.1186/s13059-016-0954-8
• Languages: English
• ISSNs: 1474-760X

Abstract

Abstract Background Mutator -like transposable elements, a class of DNA transposons, exist pervasively in both prokaryotic and eukaryotic genomes, with more than 10, 000 copies identified in the rice genome. These elements can capture ectopic genomic sequences that lead to the formation of new gene structures. Here, based on whole-genome comparative analyses, we comprehensively investigated processes and mechanisms of the evolution of putative genes derived fromMutator -like transposable elements in tenOryza species and the outgroupLeersia perieri, bridging ~20 million years of evolutionary history. Results Our analysis identified thousands of putative genes in each of theOryza species, a large proportion of which have evidence of expression and contain chimeric structures. Consistent with previous reports, we observe that the putativeMutator -like transposable element-derived genes are generally GC-rich and mainly derive from GC-rich parental sequences. Furthermore, we determine thatMutator -like transposable elements capture parental sequences preferentially from genomic regions with low methylation levels and high recombination rates. We explicitly show that methylation levels in the internal and terminated inverted repeat regions of these elements, which might be directed by the 24-nucleotide small RNA-mediated pathway, are different and change dynamically over evolutionary time. Lastly, we demonstrate that putative genes derived fromMutator -like transposable elements tend to be expressed in mature pollen, which have undergone de-methylation programming, thereby providing a permissive expression environment for newly formed/transposable element-derived genes. Conclusions Our results suggest that DNA methylation may be a primary mechanism to facilitate the origination, survival, and regulation of genes derived fromMutator -like transposable elements, thus contributing to the evolution of gene innovation and novelty in plant genomes.