Predicting the three-dimensional folding of cis-regulatory regions in mammalian genomes using bioinformatic data and polymer models. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Brackley, Chris; Brown, Jill; Waithe, Dominic; Babbs, Christian; Davies, James; Hughes, Jim; Buckle, Veronica; Marenduzzo, Davide
• Is Part Of: Genome biology. Volume 17:Issue 1(2016)
• Page Start: 1
• Page End: 16
• Publication Date: 2016-12
• Subjects: Chromosome conformation -- Polymer model -- Fluorescence in situ hybridization -- cis-regulation; Genomes -- Periodicals; Biology -- Periodicals; Molecular biology -- Periodicals; 572.8633
• DOI: 10.1186/s13059-016-0909-0
• Languages: English
• ISSNs: 1474-760X

Abstract

Abstract The three-dimensional (3D) organization of chromosomes can be probed using methods like Capture-C. However, it is unclear how such population-level data relate to the organization within a single cell, and the mechanisms leading to the observed interactions are still largely obscure. We present a polymer modeling scheme based on the assumption that chromosome architecture is maintained by protein bridges, which form chromatin loops. To test the model, we perform FISH experiments and compare with Capture-C data. Starting merely from the locations of protein binding sites, our model accurately predicts the experimentally observed chromatin interactions, revealing a population of 3D conformations.