Engineering of a high lipid producing Yarrowia lipolytica strain. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Friedlander, Jonathan; Tsakraklides, Vasiliki; Kamineni, Annapurna; Greenhagen, Emily; Consiglio, Andrew; MacEwen, Kyle; Crabtree, Donald; Afshar, Jonathan; Nugent, Rebecca; Hamilton, Maureen; Joe Shaw, A.; South, Colin; Stephanopoulos, Gregory; Brevnova, Elena
• Is Part Of: Biotechnology for biofuels. Volume 9:Issue 1(2016)
• Page Start: 1
• Page End: 12
• Publication Date: 2016-12
• Subjects: Yarrowia lipolytica -- Lipid accumulation -- Oleaginous yeast -- Metabolic engineering; Biotechnology -- Periodicals; Biomass energy -- Periodicals; Energy-Generating Resources -- Periodicals; 662.88
• DOI: 10.1186/s13068-016-0492-3
• Languages: English
• ISSNs: 1754-6834

Abstract

Abstract Background Microbial lipids are produced by many oleaginous organisms including the well-characterized yeastYarrowia lipolytica, which can be engineered for increased lipid yield by up-regulation of the lipid biosynthetic pathway and down-regulation or deletion of competing pathways. Results We describe a strain engineering strategy centered on diacylglycerol acyltransferase (DGA) gene overexpression that applied combinatorial screening of overexpression and deletion genetic targets to construct a high lipid producing yeast biocatalyst. The resulting strain, NS432, combines overexpression of a heterologous DGA1 enzyme fromRhodosporidium toruloides, a heterlogous DGA2 enzyme fromClaviceps purpurea, and deletion of the native TGL3 lipase regulator. These three genetic modifications, selected for their effect on lipid production, enabled a 77 % lipid content and 0.21 g lipid per g glucose yield in batch fermentation. In fed-batch glucose fermentation NS432 produced 85 g/L lipid at a productivity of 0.73 g/L/h. Conclusions The yields, productivities, and titers reported in this study may further support the applied goal of cost-effective, large -scale microbial lipid production for use as biofuels and biochemicals.