AdapterRemoval v2: rapid adapter trimming, identification, and read merging. (December 2016)

• Record Type: Journal Article
• Authors: Schubert, Mikkel; Lindgreen, Stinus; Orlando, Ludovic
• Is Part Of: BMC research notes. Volume 9:Number 1(2016)
• Page Start: 1
• Page End: 7
• Publication Date: 2016-12
• Subjects: Adapter identification -- Adapter trimming -- Data pre-processing -- High-throughput sequencing -- Sequence alignment; Medicine -- Periodicals; Biology -- Periodicals; 610.5
• DOI: 10.1186/s13104-016-1900-2
• Languages: English
• ISSNs: 1756-0500

Abstract

Abstract Background As high-throughput sequencing platforms produce longer and longer reads, sequences generated from short inserts, such as those obtained from fossil and degraded material, are increasingly expected to contain adapter sequences. Efficient adapter trimming algorithms are also needed to process the growing amount of data generated per sequencing run. Findings We introduce AdapterRemoval v2, a major revision of AdapterRemoval v1, which introduces(i) striking improvements in throughput, through the use of single instruction, multiple data (SIMD; SSE1 and SSE2) instructions and multi-threading support, (ii) the ability to handle datasets containing reads or read-pairs with different adapters or adapter pairs, (iii) simultaneous demultiplexing and adapter trimming, (iv) the ability to reconstruct adapter sequences from paired-end reads for poorly documented data sets, and(v) native gzip and bzip2 support. Conclusions We show that AdapterRemoval v2 compares favorably with existing tools, while offering superior throughput to most alternatives examined here, both for single and multi-threaded operations.