EV71 virus-like particles produced by co-expression of capsid proteins in yeast cells elicit humoral protective response against EV71 lethal challenge. (December 2016)

• Record Type: Journal Article
• Authors: Wang, Xiaowen; Xiao, Xiangqian; Zhao, Miao; Liu, Wei; Pang, Lin; Sun, Xin; Cen, Shan; Yang, Burton; Huang, Yuming; Sheng, Wang; Zeng, Yi
• Is Part Of: BMC research notes. Volume 9:Number 1(2016)
• Page Start: 1
• Page End: 9
• Publication Date: 2016-12
• Subjects: Enterovirus 71 -- Vaccine -- Yeast -- Virus-like particle -- Hand foot and mouth disease; Medicine -- Periodicals; Biology -- Periodicals; 610.5
• DOI: 10.1186/s13104-015-1780-x
• Languages: English
• ISSNs: 1756-0500

Abstract

Abstract Background Enterovirus 71 (EV71) is the most common causative pathogens of hand, foot and mouth disease (HFMD) associated with severe neurological complications. There is a great need to develop prophylactic vaccine against EV71 infection. Results EV71 virus-like particle (VLP) was produced in yeast expression system by the co-expression of four EV71 structural proteins VP1–VP4. Immunization with the recombinant VLPs elicited potent anti-EV71 antibody responses in adult mice and anti-VLP sera were able to neutralize EV71 virus in vitro. Neonatal mice model demonstrated VLP immunization conferred protection to suckling mice against the lethal viral challenge. Conclusions Co-expression of four EV71 structural proteins VP1–VP4 in yeast expression systems is an effective method to produce EV71 VLPs. VLP-based vaccine shows great potential to prevent EV71 infection.