Development and usefulness of an immunochromatographic device to detect antibodies for rapid diagnosis of human gnathostomiasis. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Janwan, Penchom; Intapan, Pewpan; Yamasaki, Hiroshi; Rodpai, Rutchanee; Laummaunwai, Porntip; Thanchomnang, Tongjit; Sanpool, Oranuch; Kobayashi, Kaoru; Takayama, Katsuyoshi; Kobayashi, Yukuharu; Maleewong, Wanchai
• Is Part Of: Parasites & vectors. Volume 9:Issue 1(2016)
• Page Start: 1
• Page End: 4
• Publication Date: 2016-12
• Subjects: Gnathostoma spinigerum -- Human gnathostomiasis -- Immunochromatographic test kit -- Serodiagnosis -- Recombinant protein; Parasitism -- Periodicals; Parasites -- Periodicals; Vector-pathogen relationships -- Periodicals; Animals as carriers of disease -- Periodicals; Insects as carriers of disease -- Periodicals; 616.96
• DOI: 10.1186/s13071-016-1294-y
• Languages: English
• ISSNs: 1756-3305

Abstract

Abstract Background Human gnathostomiasis is a serious tropical disease, which is often overlooked. There is an urgent need to improve tools to aid the potential diagnosis of the disease in endemic regions. To overcome this, we produced the immunochromatographic test (ICT) kit for a rapid and simple diagnosis of human gnathostomiasis. Findings The recombinant protein (named rGslic18) was applied to ICT kit as the antigen. The diagnostic value of ICT kit was evaluated using serum samples from parasitologically proven and clinically suspected gnathostomiasis patients, healthy volunteers and patients with other parasitic diseases. The ICT kit exhibited quite high sensitivity (93.75 %) and specificity (97.01 %). Conclusions The ICT kit is simple, convenient and easy to implement and expected to provide reliable diagnostic results for human gnathostomiasis. It also will be a promising diagnostic tool not only for large-scale epidemiological surveys in endemic or remote areas where diagnostic facilities are poor but also for a rapid clinical diagnosis in the bedside laboratory.