Universal conventional and real-time PCR diagnosis tools for Sarcoptes scabiei. Issue 1 (December 2015) - Angelone-Alasaad, Samer
Abstract Background The miteSarcoptes scabiei has a known host-range of over 100 mammal species including humans. One of the prime objectives of theSarcoptes -World Molecular Network (WMN) is to design and develop universalSarcoptes PCR-based diagnosis methods. Methods We describe here for the first time two universal mitochondrial-based diagnosis methods: (i) conventional end-point PCR and (ii) TaqMan real-time PCR. The design of both of these universal diagnosis methods was based onSarcoptes samples collected from 23 host species in 14 countries. Results These methods, based on skin scrapings, were successfully used to etiologically confirm the diagnosis of different clinical degrees of sarcoptic mange in 48 animals belonging to six species. These universal PCR-based diagnosis methods are highly specific, technically sensitive and simple, and are based on the amplification of 135 bp from the Mitochondrial 16S rDNA. The method based on TaqMan real-time qPCR was more sensitive than the conventional end-point PCR. Conclusions Two universal PCR-based diagnosis methods forS. scabiei were successfully designed and applied; one based on conventional end-point PCR and the other on TaqMan real-time PCR. We recommend further testing and the application of these new universal methods worldwide.