Molecular diagnosis and enrichment culture identified a septic pseudoarthrosis due to an infection with Erysipelatoclostridium ramosum. (April 2019)

• Record Type: Journal Article
• Authors: Zakham, Fathiah; Pillonel, Trestan; Brunel, Anne-Sophie; Zambelli, Pierre-Yves; Greub, Gilbert; Croxatto, Antony; Bertelli, Claire
• Is Part Of: International journal of infectious diseases. Volume 81(2019)
• Page Start: 167
• Page End: 169
• Publication Date: 2019-04
• Subjects: Clostridium ramosum -- Erysipelatoclostridium ramosum -- Pseudoarthrosis -- Graft resorption -- Bone insufficiency -- Taxogenomics -- Bacterial genomics; Communicable diseases -- Periodicals; Communicable Diseases -- Periodicals; Communicable diseases; Periodicals; Electronic journals; 616.9
• DOI: 10.1016/j.ijid.2019.02.001
• Languages: English
• ISSNs: 1201-9712

Abstract

Abstract: We describe here a rare case of septic pseudarthrosis due to Erysipelatoclostridium ramosum in a female young patient. The patient, currently in remission from Ewing's sarcoma treated by a bone resection and allograft combined with chemotherapy, suffered from a chronic femoral pseudarthrosis in a context of bone insufficiency and graft resorption. A broad range 16S PCR followed by sequencing, as well as an enrichment culture of a bone biopsy revealed the presence of E. ramosum, an anaerobic firmicute with a low Gram-positive affinity staining and low GC content, that was further characterized by whole genome sequencing (WGS).