Anti-colon cancer effect of matrix protein gene therapy with nanoparticles. (29th April 2019)

• Record Type: Journal Article
• Authors: Wang, Bilan; Yang, Daoke; Shen, Yangmei
• Is Part Of: Polymer. Volume 170(2019)
• Page Start: 148
• Page End: 156
• Publication Date: 2019-04-29
• Subjects: Matrix protein -- Cancer -- PEG-PCL-PEG; Polymers -- Periodicals; Polymerization -- Periodicals; Polymères -- Périodiques; Polymérisation -- Périodiques; 547.7
• DOI: 10.1016/j.polymer.2019.03.023
• Languages: English
• ISSNs: 0032-3861

Abstract

Abstract: The matrix protein (MP) is the core structure of the oncolytic vesicular stomatitis virus (VSV) and it played a vital role during the process of VSV infection and corresponding cytopathic effects in cancer treatment. In this study, a novel delivery synthesized by PEG-PCL-PEG and DOTAP (PPPD) was applied to encapsulate MP plasmids to derive the advantage and overcome the drawback of VSV platform in the treatment of colon cancer. The MP/DPPP micelles were stable in physiological solution, with an average diameter of 102 nm and zeta potential of 5.4 mV. This delivery system has a high encapsulation rate of 96% and transfection efficiency of 36%. A prominent anti-cancer activity of MP/DPPP was found both in Ct26 cells and animal models. The mechanisms of antitumor were further explored in this research. Our results indicated that MP/DPPP micelles were promising agents for future clinical application in the treatment of colon cancer. Graphical abstract: Image 1043 Highlights: PEG-PCL-PEG and DOTAP could assembly into nanomicelles. MP/DPPP could inhibited cancer proliferation in vitro. MP/DPPP could induced cancer apoptosis in vitro. MP/DPPP could inhibit cancer growth in vivo.