Diversity and abundance of antibiotic resistance of bacteria during the seedling period in marine fish cage-culture areas of Hainan, China. (April 2019)

• Record Type: Journal Article
• Authors: Wu, Jinjun; Mao, Can; Deng, Yiqin; Guo, Zhixun; Liu, Guangfeng; Xu, Liwen; Bei, Lei; Su, Youlu; Feng, Juan
• Is Part Of: Marine pollution bulletin. Volume 141(2019)
• Page Start: 343
• Page End: 349
• Publication Date: 2019-04
• Subjects: Antibiotic resistance genes -- Antibiotic-resistant bacteria -- Marine fish cage-culture areas -- Seedling period; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2019.02.069
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: Antibiotic resistance has become an important focus of research in the aquaculture environment. However, few studies have evaluated antibiotic resistance during the seedling period in marine fish cage-culture areas. In this study, culture-dependent methods and quantitative polymerase chain reaction were used to identify and detect cultivable heterotrophic antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs), respectively, during the seedling period in a marine fish cage-culture areas of Hainan, China. Bacterial resistance to amoxicillin, erythromycin, and gentamicin was generally high (average on 27.67%, 23.61% and 37.32%, respectively), whereas resistance to furazolidone and nitrofurantoin was generally low (average on 0.14% and 7.425%). Alteromonas (32.72%) and Vibrio (24.77%) were the dominant genus of ARB. Most ARB were opportunistic pathogens, belonging to the phylum Proteobacteria (96.02%). The abundance of sul family genes was higher than that of tet family genes. Overall, the abundance of ARGs and the resistance rates in HW was highest. Highlights: Bacterial resistance to amoxicillin, erythromycin and gentamicin was generally high. Most antibiotic-resistant bacteria (ARB) strains were opportunistic pathogens. Alteromonas was the most dominant genus of ARB. The relative abundance of sul family genes was higher than that of tet family genes.