Postnatal epigenetic regulation of intestinal stem cells requires DNA methylation and is guided by the microbiome. Issue 1 (December 2015)

• Record Type: Journal Article
• Authors: Yu, Da-Hai; Gadkari, Manasi; Zhou, Quan; Yu, Shiyan; Gao, Nan; Guan, Yongtao; Schady, Deborah; Roshan, Tony; Chen, Miao-Hsueh; Laritsky, Eleonora; Ge, Zhongqi; Wang, Hui; Chen, Rui; Westwater, Caroline; Bry, Lynn; Waterland, Robert; Moriarty, Chelsea; Hwang, Cindy; Swennes, Alton; Moore, Sean; Shen, Lanlan
• Is Part Of: Genome biology. Volume 16:Issue 1(2015)
• Page Start: 1
• Page End: 16
• Publication Date: 2015-12
• Subjects: Genomes -- Periodicals; Biology -- Periodicals; Molecular biology -- Periodicals; 572.8633
• DOI: 10.1186/s13059-015-0763-5
• Languages: English
• ISSNs: 1474-760X

Abstract

Abstract Background DNA methylation is an epigenetic mechanism central to development and maintenance of complex mammalian tissues, but our understanding of its role in intestinal development is limited. Results We use whole genome bisulfite sequencing, and find that differentiation of mouse colonic intestinal stem cells to intestinal epithelium is not associated with major changes in DNA methylation. However, we detect extensive dynamic epigenetic changes in intestinal stem cells and their progeny during the suckling period, suggesting postnatal epigenetic development in this stem cell population. We find that postnatal DNA methylation increases at 3′ CpG islands (CGIs) correlate with transcriptional activation of glycosylation genes responsible for intestinal maturation. To directly test whether 3′ CGI methylation regulates transcription, we conditionally disrupted two major DNA methyltransferases, Dnmt1 orDnmt3a, in fetal and adult intestine. Deficiency ofDnmt1 causes severe intestinal abnormalities in neonates and disrupts crypt homeostasis in adults, whereasDnmt3a loss was compatible with intestinal development. These studies reveal that 3′ CGI methylation is functionally involved in the regulation of transcriptional activation in vivo, and thatDnmt1 is a critical regulator of postnatal epigenetic changes in intestinal stem cells. Finally, we show that postnatal 3′ CGI methylation and associated gene activation in intestinal epithelial cells are significantly altered by germ-free conditions. Conclusions Our results demonstrate that the suckling period is critical for epigenetic development of intestinal stem cells, with potential important implications for lifelong gut health, and that the gut microbiome guides and/or facilitates these postnatal epigenetic processes.