Determining exon connectivity in complex mRNAs by nanopore sequencing. Issue 1 (December 2015)

• Record Type: Journal Article
• Authors: Bolisetty, Mohan; Rajadinakaran, Gopinath; Graveley, Brenton
• Is Part Of: Genome biology. Volume 16:Issue 1(2015)
• Page Start: 1
• Page End: 12
• Publication Date: 2015-12
• Subjects: Genomes -- Periodicals; Biology -- Periodicals; Molecular biology -- Periodicals; 572.8633
• DOI: 10.1186/s13059-015-0777-z
• Languages: English
• ISSNs: 1474-760X

Abstract

Abstract Short-read high-throughput RNA sequencing, though powerful, is limited in its ability to directly measure exon connectivity in mRNAs that contain multiple alternative exons located farther apart than the maximum read length. Here, we use the Oxford Nanopore MinION sequencer to identify 7, 899 'full-length' isoforms expressed from fourDrosophila genes, Dscam1, MRP, Mhc, andRdl . These results demonstrate that nanopore sequencing can be used to deconvolute individual isoforms and that it has the potential to be a powerful method for comprehensive transcriptome characterization.