Antibody-mediated inhibition of Nogo-A signaling promotes neurite growth in PC-12 cells. (28th January 2016)

• Record Type: Journal Article
• Authors: Yazdi, Iman K; Taghipour, Nima; Hmaidan, Sarah; Palomba, Roberto; Scaria, Shilpa; Munoz, Alvaro; Boone, Timothy B; Tasciotti, Ennio
• Is Part Of: Journal of tissue engineering. Volume 7(2016)
• Publication Date: 2016-01-28
• Subjects: Nogo-A -- bioactivity -- neuroregeneration -- PC-12 cells; Tissue engineering -- Periodicals; 612.028
• DOI: 10.1177/2041731416629767
• Languages: English
• ISSNs: 2041-7314

Abstract

The use of a monoclonal antibody to block the neurite outgrowth inhibitor Nogo-A has been of great interest for promoting axonal recovery as a treatment for spinal cord injury. While several cellular and non-cellular assays have been developed to quantify the bioactive effects of Nogo-A signaling, demand still exists for the development of a reliable approach to characterize the effectiveness of the anti-Nogo-A antibody. In this study, we developed and validated a novel cell-based approach to facilitate the biological quantification of a Nogo-A antibody using PC-12 cells as an in vitro neuronal cell model. Changes in the mRNA levels of the neuronal differentiation markers, growth-associated protein 43 and neurofilament light-polypeptide, suggest that activation of the Nogo-A pathway suppresses axonal growth and dendrite formation in the tested cell line. We found that application of anti-Nogo-A monoclonal antibody can significantly enhance the neuronal maturity of PC-12 cells by blocking the Nogo-A inhibitory effects, providing enhanced effects on neural maturity at the molecular level. No adverse effects were observed on cell viability.