Bisphosphonate-adsorbed ceramic nanoparticles increase bone formation in an injectable carrier for bone tissue engineering. (22nd October 2015)

• Record Type: Journal Article
• Authors: Cheng, Tegan L; Murphy, Ciara M; Ravarian, Roya; Dehghani, Fariba; Little, David G; Schindeler, Aaron
• Is Part Of: Journal of tissue engineering. Volume 6(2015)
• Publication Date: 2015-10-22
• Subjects: Bone tissue engineering -- hydroxyapatite -- tricalcium phosphate -- Bioglass 45S5 -- bisphosphonate -- recombinant human bone morphogenetic protein-2 -- sucrose acetate isobutyrate; Tissue engineering -- Periodicals; 612.028
• DOI: 10.1177/2041731415609448
• Languages: English
• ISSNs: 2041-7314

Abstract

Sucrose acetate isobutyrate (SAIB) is a sugar-based carrier. We have previously applied SAIB as a minimally invasive system for the co-delivery of recombinant human bone morphogenetic protein-2 (rhBMP-2) and found synergy when co-delivering zoledronic acid (ZA) and hydroxyapatite (HA) nanoparticles. Alternative bioceramics were investigated in a murine SAIB/rhBMP-2 injection model. Neither beta-tricalcium phosphate (TCP) nor Bioglass (BG) 45S5 had a significant effect on bone volume (BV) alone or in combination with the ZA. 14 C-labelled ZA binding assays showed particle size and ceramic composition affected binding with nano-HA > micro-HA > TCP > BG. Micro-HA and nano-HA increased BV in a rat model of rhBMP-2/SAIB injection (+278% and +337%), and BV was further increased with ZA–adsorbed micro-HA and nano-HA (+530% and +889%). These data support the use of ZA–adsorbed nanoparticle-sized HA as an optimal additive for the SAIB/rhBMP-2 injectable system for bone tissue engineering.