Trophoblast-Derived CXCL16 Decreased Granzyme B Production of Decidual γδ T Cells and Promoted Bcl-xL Expression of Trophoblasts. (April 2019)

• Record Type: Journal Article
• Authors: Fan, Deng-Xuan; Zhou, Wen-Jie; Jin, Li-Ping; Li, Ming-Qing; Xu, Xiang-Hong; Xu, Cong-Jian
• Is Part Of: Reproductive sciences. Volume 26:Number 4(2019:Apr.)
• Page Start: 532
• Page End: 542
• Publication Date: 2019-04
• Subjects: decidual γδ T cells -- trophoblasts -- CXCL16 -- CXCR6 -- unexplained recurrent spontaneous abortion; Reproductive health -- Periodicals; Reproduction -- Periodicals; 612.6
• DOI: 10.1177/1933719118777638
• Languages: English
• ISSNs: 1933-7191

Abstract

Background: Decidual γδ T cells are known to regulate the function of trophoblasts at the maternal–fetal interface; however, little is known about the molecular mechanisms of cross talk between trophoblast cells and decidual γδ T cells. Methods: Expression of chemokine C-X-C motif ligand 6 (CXCL16) and its receptor CXCR6 was evaluated in first-trimester human villus and decidual tissues by immunohistochemistry. γδ T cells were isolated from first-trimester human deciduae and cocultured with JEG3 trophoblast cells. Cell proliferation and apoptosis-related molecules, together with cytotoxicity factor and cytokine production, were measured by flow cytometry analysis. Results: Expression of CXCL16 and CXCR6 was reduced at the maternal–fetal interface in patients who experienced unexplained recurrent spontaneous abortion as compared to healthy pregnancy women. With the administration of pregnancy-related hormones or coculture with JEG3 cells, CXCR6 expression was upregulated on decidual γδ T cells. CXCL16 derived from JEG3 cells caused a decrease in granzyme B production of decidual γδ T cells. In addition, decidual γδ T cells educated by JEG3-derived CXCL16 upregulated the expression of Bcl-xL in JEG3 cells. Conclusion: This study suggested that the CXCL16/CXCR6 axis may contribute to maintaining normal pregnancy by reducing the secretion of cytotoxic factor granzyme B of decidual γδ T cells and promoting the expression of antiapoptotic marker Bcl-xL of trophoblasts.