Effect of buffer on fumarase reactions. (March 2016)

• Record Type: Journal Article
• Authors: Dunford, H. Brian
• Is Part Of: Progress in reaction kinetics and mechanism. Volume 41:Number 1(2016)
• Page Start: 23
• Page End: 28
• Publication Date: 2016-03
• Subjects: fumarate -- malate -- buffer effects -- electron density circuits -- steady state kinetics -- electron and proton transfer; Chemical kinetics -- Periodicals; 541.39405
• DOI: 10.3184/146867816X14446664644428
• Languages: English
• ISSNs: 1468-6783

Abstract

Two specific mechanisms are proposed to explain the effect of buffer on the reactions catalysed by fumarase. In one mechanism, buffer catalyses a slow isomerisation of the enzyme in which a catalytic acid group on one side of the active site is replaced by another acid group on the opposite side. In the other mechanism the buffer-catalysed isomerisation reaction is fast. The latter mechanism fits the experimental data.