High production of erythritol from Candida sorbosivorans SSE-24 and its inhibitory effect on biofilm formation of Streptococcus mutans. (December 2015)

• Record Type: Journal Article
• Authors: Saran, Saurabh; Mukherjee, Sanjana; Dalal, Jyotsana; Saxena, Rajendra Kumar
• Is Part Of: Bioresource technology. Volume 198(2015)
• Page Start: 31
• Page End: 38
• Publication Date: 2015-12
• Subjects: Erythritol -- Candida sorbosivorans SSE-24 -- RSM -- Purification -- Biofilm formation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.08.146
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Newly isolated Candida sorbosivorans SSE-24 was found to be a potent erythritol producer. Maximum erythritol of 52.32 g/L was obtained using statistical optimization. Erythritol production was successfully carried out in a 30 L fermenter. Erythritol was purified by ion exchange and crystallization to 98.99% purity. Significant inhibitory effect of erythritol on Streptococcus mutans . Abstract: Amongst different isolates screened for erythritol production, isolate no. SSE-24 was found to be the best erythritol producer and identified as Candida sorbosivorans SSE-24. Statistical optimization was used to determine the optimum level of the significant variables for maximum erythritol production. The interactive effects of glucose, inoculum level and yeast extract were determined to be significant. The optimum medium composition for erythritol production was 160 g/L glucose, 12 g/L yeast extract, 10% inoculum level and 0.35 g/L FeSO4 ⋅7H2 O. The production of erythritol was successfully scaled up to a 30 L level, where 60.20 g/L of erythritol was produced, with a yield of 0.38 g/g. The fermentation broth was purified by activated charcoal followed by vacuum concentration, ion exchange chromatography and crystallization. Purity of erythritol was further determined by NMR. Significant inhibitory effect of erythritol on growth (>78%) and biofilm formation (40.2%) of Streptococcus mutans enhances the importance of this study.