Transcriptional responses of earthworm (Eisenia fetida) exposed to naphthenic acids in soil. (September 2015)

• Record Type: Journal Article
• Authors: Wang, Jie; Cao, Xiaofeng; Sun, Jinhua; Chai, Liwei; Huang, Yi; Tang, Xiaoyan
• Is Part Of: Environmental pollution. Volume 204(2015)
• Page Start: 264
• Page End: 270
• Publication Date: 2015-09
• Subjects: Eisenia fetida -- Naphthenic acids -- ROS -- Ecotoxicity -- Quantitative real-time PCR; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2015.05.006
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: In this study, earthworms ( Eisenia fetida ) were exposed to commercial NAs contaminated soil, and changes in the levels of reactive oxygen species (ROS) and gene expressions of their defense system were monitored. The effects on the gene expression involved in reproduction and carcinogenesis were also evaluated. Significant increases in ROS levels was observed in NAs exposure groups, and the superoxide dismutase (SOD) and catalase (CAT) genes were both up-regulated at low and medium exposure doses, which implied NAs might exert toxicity by oxidative stress. The transcription of CRT and HSP70 coincided with oxidative stress, which implied both chaperones perform important functions in the protection against oxidative toxicity. The upregulation of TCTP gene indicated a potential adverse effect of NAs to terrestrial organisms through induction of carcinogenesis, and the downregulation of ANN gene indicated that NAs might potentially result in deleterious reproduction effects. Highlights: The first attempt to study gene ecotoxicity of NAs in terrestrial environment. NAs exert toxicity by oxidative stress on earthworm. NAs might cause carcinogenesis and reproductive disruption to earthworm. Abstract : NAs induced oxidative stress and altered transcriptions of genes involved in defense, reproduction, and carcinogenesis.