Conversion of crude Jatropha curcas seed oil into biodiesel using liquid recombinant Candida rugosa lipase isozymes. (September 2015)

• Record Type: Journal Article
• Authors: Kuo, Ting-Chun; Shaw, Jei-Fu; Lee, Guan-Chiun
• Is Part Of: Bioresource technology. Volume 192(2015)
• Page Start: 54
• Page End: 59
• Publication Date: 2015-09
• Subjects: Biodiesel -- Jatropha curcas -- Crude seed oil -- Candida rugosa lipase (CRL) isozyme -- Soluble lipase; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.05.008
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Recombinant CRL2 isozyme efficiently converted crude Jatropha oil into biodiesel. Optimum reaction parameters of the liquid CRL2-based process were determined. CRL2 isozyme achieved 95.3% of FAME yield in a methanol feed-batch process. Liquid CRL2 retained 53.0% of FAME yield after 3 reuse cycles for 6 days. Abstract: The versatile Candida rugosa lipase (CRL) has been widely used in biotechnological applications. However, there have not been feasibility reports on the transesterification of non-edible oils to produce biodiesel using the commercial CRL preparations, mixtures of isozymes. In the present study, four liquid recombinant CRL isozymes (CRL1–CRL4) were investigated to convert various non-edible oils into biodiesel. The results showed that recombinant CRL2 and CRL4 exhibited superior catalytic efficiencies for producing fatty acid methyl ester (FAME) from Jatropha curcas seed oil. A maximum 95.3% FAME yield was achieved using CRL2 under the optimal conditions (50 wt% water, an initial 1 equivalent of methanol feeding, and an additional 0.5 equivalents of methanol feeding at 24 h for a total reaction time of 48 h at 37 °C). We concluded that specific recombinant CRL isozymes could be excellent biocatalysts for the biodiesel production from low-cost crude Jatropha oil.