Advanced nitrogen removal via nitrite using stored polymers in a modified sequencing batch reactor treating landfill leachate. (September 2015)

• Record Type: Journal Article
• Authors: Miao, Lei; Wang, Shuying; Li, Baikun; Cao, Tianhao; Xue, Tonglai; Peng, Yongzhen
• Is Part Of: Bioresource technology. Volume 192(2015)
• Page Start: 354
• Page End: 360
• Publication Date: 2015-09
• Subjects: Landfill leachate -- Nitrogen removal -- SBR -- GAOs -- FISH; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.05.013
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Advanced nitrogen removal from landfill leachate with low C/N ratio was achieved. SBR process was modified by adding anaerobic stage after feeding to store organics. PHB and glycogen could both be the carbon source for endogenous denitritation. GAOs are the functional species for nitrogen removal, and enriched via modified SBR. The nitrite accumulation reached above 98%, which was basis of nitrogen removal. Abstracts: A modified sequencing batch reactor (SBR) operated at the anaerobic–aerobic–anoxic mode was developed in this study to fully utilize the organics in landfill leachate (ammonia concentration of 1000 ± 50 mg N/L and COD/total nitrogen (TN) ratio of 1–4). The unique feature of modified SBR process was the addition of an anaerobic stage after feeding stage, so that microorganisms could store the organics during anaerobic stage and supply the carbon source for endogenous denitritation after aeration stage. The 70-day operational tests showed the effluent TN was below 10 mg N/L at C/N ratio of 4. The intracellular stored polymers were analyzed and the microorganisms were capable of storing the carbon source as polyhydroxybutyrate (PHB) and glycogen in anaerobic stage, which were the electron donors for endogenous denitritation. Fluorescence in situ hybridization (FISH) analysis showed that glycogen accumulating organisms (GAOs) account for 39.8% of microorganisms in SBR, and carried out advanced nitrogen removal.